SUPT5H and its binding partner regulate transcriptional elongation by RNA polymerase II. SPT4 and SPT5 are involved in both 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB)-mediated transcriptional inhibition and the activation of transcriptional elongation by the human immunodeficiency virus type 1 (HIV-1) Tat protein.1) Bourgeois, C.F., et al., (2002) Mol. Cell. Biol. 22 (4), 1079-1093.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SUPT5H.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
SUPT5H antibody can be used for detection of SUPT5H by ELISA at 1:62500. SUPT5H antibody can be used for detection of SUPT5H by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
SUPT5H antibody can be used for detection of SUPT5H by ELISA at 1:62500. SUPT5H antibody can be used for detection of SUPT5H by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SUPT5H antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.