SUPT6H may be functionally analogous to SPT6 and emb-5 and may therefore regulate transcription through establishment or maintenance of chromatin structure. Spt6 may also participates in the regulation of transcription by RNA polymerase II (RNAPII). Human Spt6 (hSpt6) is a classic transcription elongation factor that enhances the rate of RNAPII elongation. HSpt6 is capable of stimulating transcription elongation both individually and in concert with DRB sensitivity-inducing factor (DSIF).1) Endoh, M., et al., (2004) Mol. Cell. Biol. 24 (8), 3324-3336.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SUPT6H.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
SUPT6H antibody can be used for detection of SUPT6H by ELISA at 1:62500. SUPT6H antibody can be used for detection of SUPT6H by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Jurkat cells at 2.5 ug/ml.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
SUPT6H antibody can be used for detection of SUPT6H by ELISA at 1:62500. SUPT6H antibody can be used for detection of SUPT6H by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SUPT6H antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt