Application
Western blot analysis of survivin in MOLT4 cell lysate in the absence (A) or presence (B) of blocking peptide with survivin antibody at 1 μg/mL.Application
Immunofluorescence of Survivin in Molt4 cells with Survivin antibody at 10 μg/mL.Application
Immunocytochemistry staining of MOLT4 cells using survivin at 10 μg/mL.| Product Name | Survivin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Survivin Antibody: API4, EPR-1, API4, IAP4, Baculoviral IAP repeat-containing protein 5, Apoptosis inhibitor 4 |
| Product description | Survivin Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel IAP protein was recently identified and designated survivin, apoptosis inhibitor 4 (API4), and TIAP. Survivin/TIAP interacted with the processed form of caspase-3 and inhibited its proteolytic activity. Survivin/TIAP is predominantly expressed in tissues of embryos, transformed cell lines, and many human cancers and lymphomas.1) Ambrosini G, Adida C, Altieri DC. A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nat Med 1997;3:917-21 |
| Immunogen | Survivin antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human survivin. The immunogen is located within the last 50 amino acids of Survivin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| Survivin antibody can be used for detection of survivin by Western blot at 1 μg/mL. An approximate 17 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BIRC5 |
|---|---|
| Alternative Names | Survivin Antibody: API4 EPR-1 API4 IAP4 Baculoviral IAP repeat-containing protein 5 Apoptosis inhibitor 4 |
| Molecular Weight(MW) | 17 kDa |
| Sequence Similarities | 2) Cat. No. 17-006 - MOLT-4 Cell Slide |
Application
Western blot analysis of survivin in MOLT4 cell lysate in the absence (A) or presence (B) of blocking peptide with survivin antibody at 1 μg/mL.
Application
Immunofluorescence of Survivin in Molt4 cells with Survivin antibody at 10 μg/mL.
Application
Immunocytochemistry staining of MOLT4 cells using survivin at 10 μg/mL.| Positive Control | 1) Cat. No. 1206 - MOLT4 Cell Lysate |
|---|---|
| Application Notes | Survivin antibody can be used for detection of survivin by Western blot at 1 μg/mL. An approximate 17 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Survivin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Survivin Antibody is supplied in PBS containing 0.02% sodium azide. |
