Application
Western blot analysis of Hemaggutinin using recombinant seasonal Hemagglutinin (lanes A & B) and swine-origin Hemagglutinin (lanes C & D) with anti-seasonal Hemagglutinin antibody (5235) at 2 μg/mL (lanes A & C) and anti-swine-origin Hemagglutinin antibody (5237) at 2 μg/mL (lanes B & D).Application
ELISA results using Swine H1N1 Hemagglutinin antibody at 1 μg/mL and the blocking and corresponding peptides at 50, 10, 2 and 0 ng/mL.| Product Name | Swine H1N1 Hemagglutinin Antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Swine H1N1 Hemagglutinin Antibody: Influenza A virus is a major public health threat, killing more than 30, 000 people per year in the USA. In early 2009, a novel swine-origin influenza A (H1N1) virus was identified in specimens obtained from patients in Mexico and the United States. The virus spread quickly around the world and on June 11, 2009, the World Health Organization declared it a pandemic. Influenza A virus has one of sixteen possible Hemagglutinin (HA) surface proteins and one of nine possible Neuraminidase (NA) surface proteins. The Hemagglutinin protein facilitates viral attachment while Neuraminidase is involved in viral release. These proteins also elicit immune responses that prevent infection or independently reduce viral replication. The genetic make-up of this swine flu virus is unlike any other: it is an H1N1 strain that combines a triple assortment first identified in 1998 including human, swine, and avian influenza with two new pig H3N2 virus genes from Eurasia, themselves of recent human origin. The distinct antigenic properties of the new swine virus compared with seasonal influenza A (H1N1) virus suggest that human immunity against new swine influenza virus is limited, although the age distribution of reported cases suggests some degree of protection in older age groups.1) Thompson WW, Shay DK, Weintraub, et al. Mortality associated with influenza and respiratory syncytial virus in the United States. JAMA2003; 289:179-186. |
| Immunogen | Hemagglutinin antibody was raised against a synthetic peptide from the novel swine influenza Hemagglutinin protein. The peptide sequence is unique from the peptide sequence for product 5241 and 5233. This antibody is a cognate pair with product 5235. The i |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| Hemagglutinin antibody can be used for the detection of the Hemagglutinin protein from the H1N1 strain of swine influenza A in ELISA. It will detect 2 ng of free peptide at 1 μg/mL. | |
| Species Reactivity | Virus |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HA |
|---|---|
| Alternative Names | Swine H1N1 Hemagglutinin Antibody: |
| Tissue Specificity | This antibody is specific for the novel swine influenza Hemagglutinin and will not recognize the corresponding Hemagglutinin sequence from the seasonal H1N1 influenza (A/Brisbane/59/2007 (H1N1)). Will not cross-react with peptide corresponding to the seasonal H1N1 Hemagglutinin. |
| Protein Accession | ACQ76314 |
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Application
Western blot analysis of Hemaggutinin using recombinant seasonal Hemagglutinin (lanes A & B) and swine-origin Hemagglutinin (lanes C & D) with anti-seasonal Hemagglutinin antibody (5235) at 2 μg/mL (lanes A & C) and anti-swine-origin Hemagglutinin antibody (5237) at 2 μg/mL (lanes B & D).
Application
ELISA results using Swine H1N1 Hemagglutinin antibody at 1 μg/mL and the blocking and corresponding peptides at 50, 10, 2 and 0 ng/mL.| Application Notes | Hemagglutinin antibody can be used for the detection of the Hemagglutinin protein from the H1N1 strain of swine influenza A in ELISA. It will detect 2 ng of free peptide at 1 μg/mL. |
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| Form | Liquid |
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| Storage Instructions | Swine H1N1 Hemagglutinin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Swine H1N1 Hemagglutinin Antibody is supplied in PBS containing 0.02% sodium azide. |
