Application
Western blot analysis of lysed extracts from JK cells untreated or treated with H2O2 for the indicated times, using syk (phospho-Tyr323).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using syk (Phospho-Tyr323). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using syk (phospho-Tyr323).| Product Name | syk (phospho Tyr323) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | p72-Syk, HSP27, HSP28, Spleen tyrosine kinase |
| Product description | Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C γdependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion1) Zhang, J. et al. (2000) J. Biol. Chem. 275, 35442-35447. Turner, M. et al. (2000) Immunol. Today 21, 148-154. Decker, M. et al. (1998) J. Biol. Chem. 273, 8867-8874. Law, C. L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315. |
| Immunogen | syk (phospho-Tyr323) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 323 (N-P-Y (p) -E- P) derived from Human syk. |
| Modification | p-Tyr323 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SYK |
|---|---|
| Alternative Names | p72-Syk HSP27 HSP28 Spleen tyrosine kinase |
| Molecular Weight(MW) | 72 kDa |
| Tissue Specificity | This antibody detects endogenous level of syk only when phosphorylated at tyrosine 323. |
| Entrez Gene | 6850 |
|---|---|
| Protein Accession | NP_001128524.1 |
Application
Western blot analysis of lysed extracts from JK cells untreated or treated with H2O2 for the indicated times, using syk (phospho-Tyr323).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using syk (Phospho-Tyr323). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using syk (phospho-Tyr323).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
