Application
Western blot analysis of extracts from HuvEc cells (Lane 2) and HepG2 cells (Lane 3), using SYK (Phospho-Tyr352) Antibody. The lane on the left is treated with antigen-specific peptide.| Product Name | SYK (phospho Tyr352) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SYK, KSYK, FLJ25043, FLJ37489 |
| Product description | Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion1) Yagi S., Biochem. Biophys. Res. Commun. 200:28-34(1994). |
| Immunogen | SYK (phospho Tyr352) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 352 (S-P-Y (p)-A-D) derived from Human SYK. |
| Modification | p-Tyr352 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SYK |
|---|---|
| Alternative Names | SYK KSYK FLJ25043 FLJ37489 |
| Molecular Weight(MW) | 72 kDa |
| Tissue Specificity | The antibody detects endogenous levels of SYK only when phosphorylated at tyrosine 352. |
| Entrez Gene | 6850 |
|---|---|
| Protein Accession | NP_001167638.1 |
Application
Western blot analysis of extracts from HuvEc cells (Lane 2) and HepG2 cells (Lane 3), using SYK (Phospho-Tyr352) Antibody. The lane on the left is treated with antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
