Application
Western blot of rat cortex lysate showing specific labeling of the ~78k synapsin protein phosphorylated at Ser 62,67 (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).| Product Name | Synapsin (phospho Ser62 / Ser67)Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Synapsin I, |
| Product description | Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Ser 549 along with Ser 62 and Ser 67 are the sites of Synapsin I that are phosphorylated by MAP kinase (Czernik et al., 1987; Jovanovic et al., 1996).1) Jovanovic JN, Benfenati, F, Siow YL, Sihra TS, Sanghera JS, Pelech SL, Greengard P,Czernik AJ (1996) Neurotrophins stimulate phosphorylation of Synapsin I by MAP kinase and regulate Synapsin I-actin interactions. Neurobiology 93:3679-3683. |
| Immunogen | Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser62, 67 of synapsin I. |
| Modification | p-Ser62 / Ser67 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with mouse, and bovine tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: |
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| Species Reactivity | BovineMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SYN1 |
|---|---|
| Alternative Names | Synapsin I |
| Molecular Weight(MW) | 78 |
Application
Western blot of rat cortex lysate showing specific labeling of the ~78k synapsin protein phosphorylated at Ser 62,67 (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).| Application Notes | The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with mouse, and bovine tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Synapsin antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
