Application
Western blot analysis of lysed extracts from Mouse Brain tissue using synapsin (Phospho-Ser9).Application
Immunofluorescence staining of methanol-fixed HeLa cells using synapsin (Phospho-Ser9).| Product Name | synapsin (phospho Ser9) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SYNI, SYN1a, SYN1b, CDKN2, |
| Product description | Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.1) Diviya Sinha, et, al. (2005) Am J Physiol Renal Physiol ; 288: F703 - F713. Franco Onofri, et, al. (2000) J. Biol. Chem ; 275: 29857. Dario Bonanomi, et, al. (2005) J. Neurosci; 25: 7299 - 7308. Hiroshi Tokumitsu, et, al. (2005) J. Biol. Chem ; 280: 35108 - 35118. |
| Immunogen | synapsin (Phospho-Ser9) antibody was raised against a peptide sequence around phosphorylation site of serine 9 (R-L-S (p) -D-S) derived from Human SYN1/synapsin. |
| Modification | p-Ser9 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SYN1 |
|---|---|
| Alternative Names | SYNI SYN1a SYN1b CDKN2 |
| Molecular Weight(MW) | 77 kDa |
| Tissue Specificity | This antibody detects endogenous level of synapsin only when phosphorylated at serine 9. |
| Entrez Gene | 6853 |
|---|---|
| Protein Accession | NP_008881.2 |
Application
Western blot analysis of lysed extracts from Mouse Brain tissue using synapsin (Phospho-Ser9).
Application
Immunofluorescence staining of methanol-fixed HeLa cells using synapsin (Phospho-Ser9).| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM292625