Application
Western blot analysis of SYNPO in rat brain tissue lysate with SYNPO antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of SYNPO in human brain tissue with SYNPO antibody at 20 μg/mL.Application
Immunohistochemistry of SYNPO in human brain tissue with SYNPO antibody at 2.5 μg/mL.| Product Name | SYNPO Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SYNPO Antibody: KIAA1029, Synaptopodin |
| Product description | SYNPO Antibody: SYNPO, also known as synaptopodin, is an actin-associated protein in telencephalic dendrites and renal podocytes. SYNPO is tightly associated with the dendritic spine apparatus, and mice lacking the SYNPO gene lack the apparatus. These SYNPO-null mice also demonstrate an impaired ability to express long-term potentiation as well as deficits in spatial memory tasks, indicating that SYNPO is involved in the regulation of synaptic plasticity. Recent studies suggest that SYNPO is linked to neuronal calcium stores and plays a role in the calcium store-associated ability of neurons to undergo long-term plasticity.1) Mundel P, Heid HW, Mundel TM, et al. Synaptopodin: an actin-associated protein in telencephalic dendrites and renal podocytes. J. Cell Biol. 1997; 139:193-204. |
| Immunogen | SYNPO antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SYNPO. The immunogen is located within the last 50 amino acids of SYNPO. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SYNPO antibody can be used for detection of SYNPO by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SYNPO |
|---|---|
| Alternative Names | SYNPO Antibody: KIAA1029 Synaptopodin |
| Molecular Weight(MW) | Predicted: 75, 99, 102 kDa Observed: 95 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least three isoforms of SYNPO are known to exist; this antibody will detect all three isoforms. SYNPO antibody is predicted to not cross-react with other SYNPO family member proteins. |
| Entrez Gene | 11346 |
|---|---|
| Protein Accession | NP_001159680 |
Application
Western blot analysis of SYNPO in rat brain tissue lysate with SYNPO antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of SYNPO in human brain tissue with SYNPO antibody at 20 μg/mL.
Application
Immunohistochemistry of SYNPO in human brain tissue with SYNPO antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | SYNPO antibody can be used for detection of SYNPO by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SYNPO antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SYNPO Antibody is supplied in PBS containing 0.02% sodium azide. |
