Application
Western blot analysis in A549 cell line lysates (35ug/lane).Application
TAGAP antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TAGAP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | T-cell activation Rho GTPase-activating protein, T-cell activation GTPase-activating protein, TAGAP, TAGAP1 |
| Product description | This gene encodes a protein that may function as a Rho GTPase-activating protein. Transcript variants encoding distinct isoforms have been identified.1) Hinks, A., et al. Ann. Rheum. Dis. 69(12):2169-2172(2010) |
| Immunogen | This TAGAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-129 amino acids from the Central region of human TAGAP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TAGAP |
|---|---|
| Alternative Names | T-cell activation Rho GTPase-activating protein T-cell activation GTPase-activating protein TAGAP TAGAP1 |
| Molecular Weight(MW) | 81 kDa |
Application
Western blot analysis in A549 cell line lysates (35ug/lane).
Application
TAGAP antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
