Application
Western blot analysis of TANK in Daudi cell lysate with TANK antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunocytochemistry of TANK in Daudi cells with TANK antibody at 2.5 μg/mL.| Product Name | TANK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TANK Antibody: ITRAF, TRAF2, I-TRAF, ITRAF, TRAF family member-associated NF-kappa-B activator, TRAF-interacting protein |
| Product description | TANK Antibody: TANK was initially identified as a novel TRAF-interacting protein that regulated TRAF-mediated signal transduction. Specifically, ligand binding by surface receptors in the tumor necrosis factor (TNF) receptor and Toll/interleukin-1 (IL-1) receptor families lead to the formation of a TRAF/TANK complex that mediates the activation of the transcription factor NF-κB. This activation of NF-κB occurs through an association with the kinases IKKε and TBK1. More recently, it was shown that these proteins can then form a complex with NEMO, a protein that regulates the activity of the IκB complex. This suggests that in addition to the possibility that TBK1 and IKKε activate the IKKs, the association with the IKK complex may help these kinases modulate other functions, such as the transactivation potential of NF-κB proteins. At least two isoforms of TANK are known to exist.1) Cheng G and Baltimore D. TANK, a co-inducer with TRAF2 of TNF- and CD40L-mediated NF-κB activation. Genes Dev. 1996; 10:963-73. |
| Immunogen | TANK antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human TANK. The immunogen is located within amino acids 60 - 110 of TANK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| TANK antibody can be used for the detection of TANK by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TANK |
|---|---|
| Alternative Names | TANK Antibody: ITRAF TRAF2 I-TRAF ITRAF TRAF family member-associated NF-kappa-B activator TRAF-interacting protein |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (79%) |
Application
Western blot analysis of TANK in Daudi cell lysate with TANK antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunocytochemistry of TANK in Daudi cells with TANK antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | TANK antibody can be used for the detection of TANK by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TANK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TANK Antibody is supplied in PBS containing 0.02% sodium azide. |
