Application
To detect hTARC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTARC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hTARC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTARC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.| Product Name | Tarc Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TARC, ABCD-2, SCYA17, A-152E5.3, TARC, C-C motif chemokine 17, CC chemokine TARC, |
| Product description | Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. TARC displays chemotactic activity for T lymphocytes, but not monocytes or granulocytes. TARC binds to chemokine receptors CCR4 and CCR8. This chemokine plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTARC (human Thymus and Activation Regulated Chemokine). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hTARC (100.00 ng/mL), a concentration of 20.0 - 30 μg/mL of this antibody is required. ELISA: To detect hTARC by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTARC. Sandwich: To detect hTARC by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TARC (XP-5279Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTARC. Western Blot: To detect hTARC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTARC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCL17 |
|---|---|
| Alternative Names | TARC ABCD-2 SCYA17 A-152E5.3 TARC C-C motif chemokine 17 CC chemokine TARC |
Application
To detect hTARC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTARC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hTARC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTARC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hTARC (100.00 ng/mL), a concentration of 20.0 - 30 μg/mL of this antibody is required. ELISA: To detect hTARC by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTARC. Sandwich: To detect hTARC by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TARC (XP-5279Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTARC. Western Blot: To detect hTARC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTARC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Tarc antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
