Application
Western blot analysis of lysed extracts from mouse brain tissue using Tau (Phospho-Ser404).Application
Top Image: Immunohistochemical analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer’s Disease using Tau (Phospho-Ser404). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Tau (Phospho-Ser404).| Product Name | Tau (phospho Ser404) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TAU, MSTD, PPND, DDPAC, MAPTL, MTBT1, MTBT2, FTDP-17, JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1, MAPT, MTAPT, Neurofibrillary tangle protein, PHF-tau |
| Product description | Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.1) Li G, Yin H, et. al . (2004) J Biol Chem ; 279 (16): 15938-45. Noble W, et al. (2003) Neuron ; 38 (4): 555-65. Giasson BI, et al. (2002) . Biochemistry; 41 (51): 15376-87. Lee G., et. al. (1989) . Neuron 2:1615-1624. Andreadis A. et. al. (1992) Biochemistry 31:10626-10633. |
| Immunogen | Tau (Phospho-Ser404) antibody was raised against a peptide sequence around phosphorylation site of serine 404 (D-T-S (p) -P-R) derived from Human Tau. |
| Modification | p-Ser404 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPT |
|---|---|
| Alternative Names | TAU MSTD PPND DDPAC MAPTL MTBT1 MTBT2 FTDP-17 JNKK1 MEK4 MKK4 PRKMK4 SEK1 SERK1 SKK1 MAPT MTAPT Neurofibrillary tangle protein PHF-tau |
| Molecular Weight(MW) | 48, 62, 78 kDa |
| Tissue Specificity | This antibody detects endogenous level of Tau only when phosphorylated at serine 404. |
| Entrez Gene | 4137 |
|---|---|
| Protein Accession | NP_001116538.1 |
Application
Western blot analysis of lysed extracts from mouse brain tissue using Tau (Phospho-Ser404).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer’s Disease using Tau (Phospho-Ser404). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Tau (Phospho-Ser404).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
