Application
Western blot analysis of TBC1D1 in Daudi cell lysate with TBC1D1 antibody at (A) 1, (B) 2 and (C) 4 μg/mL.| Product Name | TBC1D1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TBC1D1 Antibody: TBC, TBC1, KIAA1108, TBC1 domain family member 1 |
| Product description | TBC1D1 Antibody: TBC1D1 is the founding member of a family of proteins sharing a 180- to 200-amino acid TBC domain and presumed to have a role in regulating cell growth and differentiation. These proteins share significant homology with TRE2/USP6, yeast Bub2, and CDC16. TBC1D1 and TBC1D4 (AS160) have been demonstrated to be Rab GAPs (GTPase-activating proteins) that link upstream to Akt and phosphoinositide 3-kinase and downstream to Rabs involved in trafficking of GLUT4 vesicles. TBC1D1 regulates insulin-mediated GLUT4 translocation through its GAP activity, and is a likely Akt substrate. Mutations in the Tbc1d1 gene lead to some cases of severe human obesity.1) White RA, Pasztor LM, Richardson PM, et al. The gene encoding TBC1D1 with homology to the tre-2/USP6 oncogene, Bub2, and cdc16 maps to mouse chromosome 5 and human chromosome 4. Cytogenet. Cell Genet.2000; 89:272-5. |
| Immunogen | TBC1D1 antibody was raised against a 22 amino acid synthetic peptide from near the carboxy terminus of human TBC1D1. The immunogen is located within the last 50 amino acids of TBC1D1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TBC1D1 antibody can be used for detection of TBC1D1 by Western blot at 1 - 4 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TBC1D1 |
|---|---|
| Alternative Names | TBC1D1 Antibody: TBC TBC1 KIAA1108 TBC1 domain family member 1 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (71%) |
Application
Western blot analysis of TBC1D1 in Daudi cell lysate with TBC1D1 antibody at (A) 1, (B) 2 and (C) 4 μg/mL.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | TBC1D1 antibody can be used for detection of TBC1D1 by Western blot at 1 - 4 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TBC1D1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TBC1D1 Antibody is supplied in PBS containing 0.02% sodium azide. |
