Application
Antibody used in WB on Human Placenta at 2.5 ug/ml.| Product Name | TBP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TBP, HDL4, GTF2D, SCA17, TFIID, GTF2D1 |
| Product description | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.1) Reid, S.J., et al., (2004) Brain Res. Mol. Brain Res. 125 (1-2), 120-128. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TBP. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TBP antibody can be used for detection of TBP by ELISA at 1:62500. TBP antibody can be used for detection of TBP by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TBP |
|---|---|
| Alternative Names | TBP HDL4 GTF2D SCA17 TFIID GTF2D1 |
| Molecular Weight(MW) | 38 kDa |
Application
Antibody used in WB on Human Placenta at 2.5 ug/ml.| Positive Control | 1) Cat. No. 1309 - Human Placenta Lysate |
|---|---|
| Application Notes | TBP antibody can be used for detection of TBP by ELISA at 1:62500. TBP antibody can be used for detection of TBP by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store TBP antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
