Application
Fluorescent image of HUVEC cell stained with TBX15 Antibody (N-term).HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX15 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated PhalloidApplication
Western blot analysis in mouse liver tissue lysates (35ug/lane).| Product Name | TBX15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | T-box transcription factor TBX15, T-box protein 15, T-box transcription factor TBX14, T-box protein 14, TBX15, TBX14 |
| Product description | Probable transcriptional regulator involved in developmental processes. |
| Immunogen | This TBX15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-73 amino acids from the N-terminal region of human TBX15. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For IF starting dilution is: 1:10~50 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TBX15 |
|---|---|
| Alternative Names | T-box transcription factor TBX15 T-box protein 15 T-box transcription factor TBX14 T-box protein 14 TBX15 TBX14 |
| Molecular Weight(MW) | 66 kDa |
Application
Fluorescent image of HUVEC cell stained with TBX15 Antibody (N-term).HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX15 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloid
Application
Western blot analysis in mouse liver tissue lysates (35ug/lane).| Application Notes | For IF starting dilution is: 1:10~50 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
