TBX5 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs.1) Zaragoza, M.V., et al., () Gene 330, 38613 2004.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TBX5.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
TBX5 antibody can be used for detection of TBX5 by ELISA at 1:62500. TBX5 antibody can be used for detection of TBX5 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in IHC on Human muscle at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human Jurkat 0.5 ug/ml.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
TBX5 antibody can be used for detection of TBX5 by ELISA at 1:62500. TBX5 antibody can be used for detection of TBX5 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TBX5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.