Application
Antibody used in IHC on Human Brain, cortex at 5.0 ug/ml.Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | TBX5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TBX5, HOS |
| Product description | TBX5 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. TBX5 is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in TBX5 have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for TBX5.1) Zaragoza, M.V., et al., (2004) Gene 330, 38978. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TBX5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TBX5 antibody can be used for detection of TBX5 by ELISA at 1:62500. TBX5 antibody can be used for detection of TBX5 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TBX5 |
|---|---|
| Alternative Names | TBX5 HOS |
| Molecular Weight(MW) | 58 kDa, 60 kDa, 53 kDa, 39 kDa, 58 kDa |
Application
Antibody used in IHC on Human Brain, cortex at 5.0 ug/ml.
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | TBX5 antibody can be used for detection of TBX5 by ELISA at 1:62500. TBX5 antibody can be used for detection of TBX5 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store TBX5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
