Application
Western blot analysis in ZR-75-1 cell line and mouse spleen tissue lysates (35ug/lane).Application
Fluorescent confocal image of Hela cell stained with TBX6 Antibody . Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX6 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin| Product Name | TBX6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | T-box transcription factor TBX6, T-box protein 6, TBX6 |
| Product description | This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. Knockout studies in mice indicate that this gene is important for specification of paraxial mesoderm structures.1) Fei, Q., et al. Spine 35(9):983-988(2010) |
| Immunogen | This TBX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-293 amino acids from the Central region of human TBX6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TBX6 |
|---|---|
| Alternative Names | T-box transcription factor TBX6 T-box protein 6 TBX6 |
| Molecular Weight(MW) | 47 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in ZR-75-1 cell line and mouse spleen tissue lysates (35ug/lane).
Application
Fluorescent confocal image of Hela cell stained with TBX6 Antibody . Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX6 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
