Application
Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunocytochemistry of TCF12 in HeLa cells with TCF12 antibody at 10 μg/mL.| Product Name | TCF12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TCF12 Antibody: HEB, CRS3, HTF4, bHLHb20, HsT17266, BHLHB20, HEB, Transcription factor 12, Class B basic helix-loop-helix protein 20, TCF-12 |
| Product description | TCF12 Antibody: TCF12, also known as HTF4, is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. TCF12 is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. TCF12, in combination with E2A, is required to block thymocyte proliferation prior to pre-TCR expression and is critical for proper T cell differentiation. Recent reports have shown that TCF12 is also a critical factor required for the development of invariant natural killer T cells.1) Zhang Y and Bina M. The nucelotide sequence of the human transcription factor HTF4a cDNA. DNA Seq.1992; 2:397-403. |
| Immunogen | TCF12 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TCF12. The immunogen is located within amino acids 50 - 100 of TCF12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| TCF12 antibody can be used for detection of TCF12 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TCF12 |
|---|---|
| Alternative Names | TCF12 Antibody: HEB CRS3 HTF4 bHLHb20 HsT17266 BHLHB20 HEB Transcription factor 12 Class B basic helix-loop-helix protein 20 TCF-12 |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunocytochemistry of TCF12 in HeLa cells with TCF12 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
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| Application Notes | TCF12 antibody can be used for detection of TCF12 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TCF12 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TCF12 Antibody is supplied in PBS containing 0.02% sodium azide. |
