Application
Western blot analysis of lysates from Hela cell line mouse spleen tissue lysate(from left to right), using TCF21 Antibody at 1:1000 at each lane.Application
Western blot analysis in 293 cell line and human placenta tissue lysates (35ug/lane).Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TCF21 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Transcription factor 21, TCF-21, Capsulin, Class A basic helix-loop-helix protein 23, bHLHa23, Epicardin, Podocyte-expressed 1, Pod-1, TCF21, BHLHA23, POD1 |
| Product description | TCF21 encodes a transcription factor of the basic helix-loop-helix family. The TCF21 product is mesoderm specific, and expressed in embryonic epicardium, mesenchyme-derived tissues of lung, gut, gonad, and both mesenchymal and glomerular epithelial cells in the kidney. Two transcript variants encoding the same protein have been found for this gene.1) Jugessur, A., et al. PLoS ONE(7), E11493 (2010) : |
| Immunogen | This TCF21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-173 amino acids from the C-terminal region of human TCF21. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TCF21 |
|---|---|
| Alternative Names | Transcription factor 21 TCF-21 Capsulin Class A basic helix-loop-helix protein 23 bHLHa23 Epicardin Podocyte-expressed 1 Pod-1 TCF21 BHLHA23 POD1 |
| Molecular Weight(MW) | 20 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of lysates from Hela cell line mouse spleen tissue lysate(from left to right), using TCF21 Antibody at 1:1000 at each lane.
Application
Western blot analysis in 293 cell line and human placenta tissue lysates (35ug/lane).
Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM292978