TCF25 acts as a transcriptional repressor. It has been shown to repress transcription of SRF in vitro and so may play a role in heart development.1) Steen, H. (2008) Biochem. Biophys. Res. Commun. 366 (2), 432-437.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TCF25.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
TCF25 antibody can be used for detection of TCF25 by ELISA at 1:62500. TCF25 antibody can be used for detection of TCF25 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.
Positive Control
1) Tranfected 293T Cell Lysate
Application Notes
TCF25 antibody can be used for detection of TCF25 by ELISA at 1:62500. TCF25 antibody can be used for detection of TCF25 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TCF25 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.