Application
Western blot analysis of TCF3 in Human brain tissue lysate with TCF3 antibody at 1 μg/mL in (A) the absence and (B) presence of peptide blocking.Application
Immunofluorescence of TCF3 in rat brain tissue with TCF3 antibody at 20 μg/mL.Application
Immunohistochemistry of TCF3 in rat liver tissue with TCF3 antibody at 5 μg/mL.| Product Name | TCF3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TCF3 Antibody: E2A, E47, ITF1, VDIR, TCF-3, bHLHb21, BHLHB21, E2A, Transcription factor E2-alpha, Class B basic helix-loop-helix protein 21 |
| Product description | TCF3 Antibody: The TCF3 gene, also called E2A, encodes two basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. These transcription factors are involved in mediating canonical Wnt signaling, which is very important in a diverse array of cellular functions such as stem cell proliferation, self-renewal, activation, fate determination, differentiation and aging and senescence. They bind beta-catenin and can act as transcriptional activators or repressors for Wnt target genes, and have been shown to regulate specific target genes during CNS development downstream of Wnt signaling. TCF3/Lef complexes are also known to play key roles in controlling cell fate lineages in multipotent skin stem cells.1) Korinek V, Barker N, Willert K, et al. Two members of the Tcf family implicated in Wnt/beta-catenin signaling during embryogenesis in the mouse. Mol. Cell Biol.1998; 18:1248-1256. |
| Immunogen | TCF3 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human TCF3. The immunogen is located within amino acids 150 - 200 of TCF3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TCF3 antibody can be used for detection of TCF3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TCF3 |
|---|---|
| Alternative Names | TCF3 Antibody: E2A E47 ITF1 VDIR TCF-3 bHLHb21 BHLHB21 E2A Transcription factor E2-alpha Class B basic helix-loop-helix protein 21 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken: (86%) |
Application
Western blot analysis of TCF3 in Human brain tissue lysate with TCF3 antibody at 1 μg/mL in (A) the absence and (B) presence of peptide blocking.
Application
Immunofluorescence of TCF3 in rat brain tissue with TCF3 antibody at 20 μg/mL.
Application
Immunohistochemistry of TCF3 in rat liver tissue with TCF3 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | TCF3 antibody can be used for detection of TCF3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TCF3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TCF3 Antibody is supplied in PBS containing 0.02% sodium azide. |
