Application
Antibody used in WB on Human HepG2 cells at 1 ug/ml.| Product Name | TCF3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TCF3, E2A, E47, ITF1, VDIR, TCF-3, bHLHb21 |
| Product description | TCF3 contains 1 basic helix-loop-helix (bHLH) domain. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. TCF3 binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.1) Maruyama, K. (2005) J. Biol. Chem. 280 (41), 34577-34589. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TCF3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TCF3 antibody can be used for detection of TCF3 by ELISA at 1:1562500. TCF3 antibody can be used for detection of TCF3 by western blot at 0.31 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TCF3 |
|---|---|
| Alternative Names | TCF3 E2A E47 ITF1 VDIR TCF-3 bHLHb21 |
| Molecular Weight(MW) | 68 kDa |
Application
Antibody used in WB on Human HepG2 cells at 1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | TCF3 antibody can be used for detection of TCF3 by ELISA at 1:1562500. TCF3 antibody can be used for detection of TCF3 by western blot at 0.31 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store TCF3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
