Tryptophan 2,3-dioxygenase (EC 1.13.11.11) plays a role in catalyzing the first and rat-limiting step in the kynurenine pathway, the major pathway of tryptophan metabolism.1) Basran, J., (2008) Biochemistry 47 (16), 4752-4760.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TDO2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
TDO2 antibody can be used for detection of TDO2 by ELISA at 1:312500. TDO2 antibody can be used for detection of TDO2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Positive Control
1) Cat. No. XBL-10407 - Fetal Heart Tissue Lysate
Application Notes
TDO2 antibody can be used for detection of TDO2 by ELISA at 1:312500. TDO2 antibody can be used for detection of TDO2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TDO2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.