Application
Western Blot at 1:2000 dilution + human cerebellum lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis in mouse liver tissue lysates (35ug/lane).| Product Name | TEF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Thyrotroph embryonic factor, TEF, KIAA1655 |
| Product description | Thyrotroph embryonic factor (TEF), a transcription factor, is a member of the PAR (proline and acidic amino acid-rich) subfamily of basic region/leucine zipper (bZIP) transcription factors. It is expressed in a broad range of cells and tissues in adult animals, however, during embryonic development, TEF expression appears to be restricted to the developing anterior pituitary gland, coincident with the appearance of thyroid-stimulating hormone, beta (TSHB). Indeed, TEF can bind to, and transactivate the TSHB promoter. It shows homology (in the functional domains) with other members of the PAR-bZIP subfamily of transcription factors, which include albumin D box-binding protein (DBP), human hepatic leukemia factor (HLF) and chicken vitellogenin gene-binding protein (VBP); VBP is considered the chicken homologue of TEF. Different members of the subfamily can readily form heterodimers, and share DNA-binding, and transcriptional regulatory properties. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].1) Kripke, D.F., et al. Psychiatry Investig 7(1):36-42(2010) |
| Immunogen | This TEF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 213-240 amino acids from the C-terminal region of human TEF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TEF |
|---|---|
| Alternative Names | Thyrotroph embryonic factor TEF KIAA1655 |
| Molecular Weight(MW) | 33 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
Western Blot at 1:2000 dilution + human cerebellum lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis in mouse liver tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
