Application
Western blot analysis of TEM5 in rat kidney tissue lysate with TEM5 antibody at (A) 1 μg/ml and (B)2 μg/mL.Application
Immunofluorescence of TEM5 in human bladder tissue with TEM5 antibody at 20 μg/mL.Application
Immunohistochemistry of TEM5 in human bladder tissue with TEM5 antibody at 5 μg/mL.| Product Name | TEM5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TEM5 Antibody: TEM5, KIAA1531, TEM5, G-protein coupled receptor 124, Tumor endothelial marker 5 |
| Product description | TEM5 Antibody: Tumor endothelial markers (TEMs) are significantly up-regulated during angiogenesis and neoangiogensis that are crucial for the growth of solid tumors. TEMs localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies. These include TEMs such as TEM1, TEM5, TEM7 and TEM8. TEM5 is a member of the adhesion family of G protein coupled receptors and is localized on the surface of endothelial cells. TEM5 is a seven-pass transmembrane receptor, unlike TEM1, TEM7 and TEM8 which span the membrane once. TEM5 is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. Proteolytically processed soluble TEM5 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans.1) Carson-Walter EB, Watkins DN, Nanda A, et al. Cell surface tumor endothelial markers are conserved in mice and humans. Cancer Res.2001; 61:6649-55. |
| Immunogen | TEM5 antibody was raised against a 14 amino acid synthetic peptide near the center of human TEM5. The immunogen is located within amino acids 560 - 610 of TEM5. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| WB:1:500~1:2000 IF:1:50~1:100 IHC:1:50~1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GPR124 |
|---|---|
| Alternative Names | TEM5 Antibody: TEM5 KIAA1531 TEM5 G-protein coupled receptor 124 Tumor endothelial marker 5 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (85%) |
Application
Western blot analysis of TEM5 in rat kidney tissue lysate with TEM5 antibody at (A) 1 μg/ml and (B)2 μg/mL.
Application
Immunofluorescence of TEM5 in human bladder tissue with TEM5 antibody at 20 μg/mL.
Application
Immunohistochemistry of TEM5 in human bladder tissue with TEM5 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1465 - Rat Kidney Tissue Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 IF:1:50~1:100 IHC:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | TEM5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TEM5 Antibody is supplied in PBS containing 0.02% sodium azide. |
