Application
Western Blot at 1:2000 dilution Lane 1: Raji whole cell lysate Lane 2: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis in Jurkat cell line and human normal uterus tissue lysates (35ug/lane).This demonstrdetected the TERT protein (arrow).Application
Formalin-fixed and paraffin-embedded human lymph with TERT Antibody (S1125), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Confocal immunofluorescent analysis of TERT Antibody (S1125) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
Flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TERT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Telomerase reverse transcriptase, HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, TP2, TERT, EST2, TCS1, TRT |
| Product description | Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks.1) Sekaric,P., J. Virol. 82 (1), 71-76 (2008) Okawa,T., Genes Dev. 21 (21), 2788-2803 (2007) |
| Immunogen | This TERT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1104-1132 amino acids from human TERT. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TERT |
|---|---|
| Alternative Names | Telomerase reverse transcriptase HEST2 Telomerase catalytic subunit Telomerase-associated protein 2 TP2 TERT EST2 TCS1 TRT |
| Molecular Weight(MW) | 127 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: Raji whole cell lysate Lane 2: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis in Jurkat cell line and human normal uterus tissue lysates (35ug/lane).This demonstrdetected the TERT protein (arrow).
Application
Formalin-fixed and paraffin-embedded human lymph with TERT Antibody (S1125), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Confocal immunofluorescent analysis of TERT Antibody (S1125) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
Flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
