Application
Western blot analysis in Hela,HL-60 cell line lysates (35ug/lane).Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TFAP4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Transcription factor AP-4, Activating enhancer-binding protein 4, Class C basic helix-loop-helix protein 41, bHLHc41, TFAP4, BHLHC41 |
| Product description | Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al., 1988 [PubMed 2833704]; Hu et al., 1990 [PubMed 2123466]).1) Ku, W.C., et al. Mol. Cell Proteomics 8(9):2034-2050(2009) |
| Immunogen | This TFAP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-154 amino acids from the Central region of human TFAP4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TFAP4 |
|---|---|
| Alternative Names | Transcription factor AP-4 Activating enhancer-binding protein 4 Class C basic helix-loop-helix protein 41 bHLHc41 TFAP4 BHLHC41 |
| Molecular Weight(MW) | 39 kDa |
Application
Western blot analysis in Hela,HL-60 cell line lysates (35ug/lane).
Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
