TFB2M is a S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. It is also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. It stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity.1) Falkenberg, M., (2002) Nat. Genet. 31 (3), 289-294.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TFB2M.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
TFB2M antibody can be used for detection of TFB2M by ELISA at 1:312500. TFB2M antibody can be used for detection of TFB2M by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Transfected 293T at 1.25 ug/ml.
Positive Control
1) Tranfected 293T Cell Lysate
Application Notes
TFB2M antibody can be used for detection of TFB2M by ELISA at 1:312500. TFB2M antibody can be used for detection of TFB2M by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TFB2M antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt