Application
Western blot analysis of TFF3 in human colon tissue lysate with TFF3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of TFF3 in Human Small Intestine tissue with TFF3 antibody at 20 μg/mL.Application
Immunohistochemistry of TFF3 in human small intestine tissue with TFF3 antibody at 2.5 μg/mL.| Product Name | TFF3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TFF3 Antibody: ITF, P1B, TFI, ITF, Trefoil factor 3, Intestinal trefoil factor, hITF |
| Product description | TFF3 Antibody: Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. Trefoil peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain and clustered in human chromosome 21q22.3. Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes in the large and small intestines and lower respiratory tract. TFF3 is mainly active as a disulfide-linked homodimer and may have a role in promoting cell migration in healing processes. It is involved in the maintenance and repair of the intestinal mucosa.1) Podolsky DK, Lynch-Devaney K, Stow JL, et al. Identification of human intestinal trefoil factor. Goblet cell-specific expression of a peptide targeted for apical secretion. J. Biol. Chem. 1993; 268:6694-702. |
| Immunogen | TFF3 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human TFF3. The immunogen is located within the last 50 amino acids of TFF3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TFF3 antibody can be used for detection of TFF3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TFF3 |
|---|---|
| Alternative Names | TFF3 Antibody: ITF P1B TFI ITF Trefoil factor 3 Intestinal trefoil factor hITF |
| Molecular Weight(MW) | Predicted: 10 kDa Observed: 11 kDa |
| Sequence Similarities | 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
| Tissue Specificity | TFF3 antibody is predicted to not cross-react with other TFF family members. |
Application
Western blot analysis of TFF3 in human colon tissue lysate with TFF3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of TFF3 in Human Small Intestine tissue with TFF3 antibody at 20 μg/mL.
Application
Immunohistochemistry of TFF3 in human small intestine tissue with TFF3 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1320 - Human Colon Tissue Lysate |
|---|---|
| Application Notes | TFF3 antibody can be used for detection of TFF3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TFF3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TFF3 Antibody is supplied in PBS containing 0.02% sodium azide. |
