Application
Western blot analysis of TGIF1 using rabbit polyclonal using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TGIF1 gene.Application
Western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the TGIF1 antibody detected the TGIF1 protein (arrow).| Product Name | TGIF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Homeobox protein TGIF1, 5'-TG-3'-interacting factor 1, TGIF1, TGIF |
| Product description | The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and eight variants, encoding four distinct isoforms, are described.1) Bengoechea-Alonso, M.T., et al. Oncogene 29(38):5322-5328(2010) |
| Immunogen | This TGIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 208-237 amino acids from the Central region of human TGIF1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | TGIF1 |
|---|---|
| Alternative Names | Homeobox protein TGIF1 5'-TG-3'-interacting factor 1 TGIF1 TGIF |
| Molecular Weight(MW) | 43 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
Western blot analysis of TGIF1 using rabbit polyclonal using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TGIF1 gene.
Application
Western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the TGIF1 antibody detected the TGIF1 protein (arrow).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
