Application
Western blot analysis of lysates from SW480, U-87 MG cell line (from left to right), using TH Antibody at 1:1000 at each lane.Application
Western blot analysis in SH-SY5Y cell line lysates (35ug/lane).This demonstrates the detected the TH protein (arrow).Application
Formalin-fixed and paraffin-embedded human brain tissue with TH Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of K562 cells using TH Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | TH is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons.1) Kuhn,D.M., et.al., J. Biol. Chem. 277 (16), 14336-14342 (2002) |
| Immunogen | This TH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 486-514 amino acids from the C-terminal region of human TH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TH |
|---|---|
| Alternative Names | Tyrosine 3-monooxygenase Tyrosine 3-hydroxylase TH TH TYH |
| Molecular Weight(MW) | 59 kDa |
Application
Western blot analysis of lysates from SW480, U-87 MG cell line (from left to right), using TH Antibody at 1:1000 at each lane.
Application
Western blot analysis in SH-SY5Y cell line lysates (35ug/lane).This demonstrates the detected the TH protein (arrow).
Application
Formalin-fixed and paraffin-embedded human brain tissue with TH Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of K562 cells using TH Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
