Application
Western blot analysis of anti-THY1 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide (BP2050a) in T47D cell line lysate.Application
Antibody is used in Western blot to detect THY1 in HL60 cell lysate.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with THY1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with THY1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | THY1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Thy-1 membrane glycoprotein, CDw90, Thy-1 antigen, CD90, THY1 |
| Product description | THY1 (CD90) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain.1) Koumas, L., et al., Am. J. Pathol. 163(4):1291-1300 (2003). Abeysinghe, H.R., et al., Cancer Genet. Cytogenet. 143(2):125-132 (2003). Saalbach, A., et al., Microvasc. Res. 64(1):86-93 (2002). Henniker, A.J., J. Biol. Regul. Homeost. Agents 15(4):392-393 (2001). Ye, Z., et al., Biochem. Biophys. Res. Commun. 275(1):223-227 (2000). |
| Immunogen | This THY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-65 amino acids from the N-terminal region of human THY1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | THY1 |
|---|---|
| Alternative Names | Thy-1 membrane glycoprotein CDw90 Thy-1 antigen CD90 THY1 |
| Molecular Weight(MW) | 18 kDa |
Application
Western blot analysis of anti-THY1 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide (BP2050a) in T47D cell line lysate.
Application
Antibody is used in Western blot to detect THY1 in HL60 cell lysate.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with THY1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with THY1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
