Application
Western blot analysis of TIMP3 Antibody in HepG2 cell line lysates (35ug/lane)Application
Western blot analysis of TIMP3 Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse NIH-3T3 cell line lysate.Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TIMP3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TIMP3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Metalloproteinase inhibitor 3, Protein MIG-5, Tissue inhibitor of metalloproteinases 3, TIMP-3, TIMP3 |
| Product description | TIMP3 is one of inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM).1) Jacobson,S.G., et.al., Nat. Genet. 11 (1), 27-32 (1995) Wick,M., et.al., Biochem. J. 311 (PT 2), 549-554 (1995) |
| Immunogen | This TIMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-139 amino acids from the Central region of human TIMP3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TIMP3 |
|---|---|
| Alternative Names | Metalloproteinase inhibitor 3 Protein MIG-5 Tissue inhibitor of metalloproteinases 3 TIMP-3 TIMP3 |
| Molecular Weight(MW) | 24 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of TIMP3 Antibody in HepG2 cell line lysates (35ug/lane)
Application
Western blot analysis of TIMP3 Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse NIH-3T3 cell line lysate.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TIMP3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
