Application
Western blot analysis of TLR1 in mouse spleen lysate with TLR1 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.Application
Immunofluorescence of TLR1 in Mouse Spleen tissueApplication
Immunohistochemistry of TLR1 in mouse spleen tissue with TLR1 antibody at 10 μg/mL.| Product Name | TLR1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TLR1 Antibody: TIL, CD281, rsc786, TIL. LPRS5, KIAA0012, Toll-like receptor 1, TIL |
| Product description | TLR1 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR1 is co-expressed with TLR2 on myeloid cells of the innate immune systems in lymphoid tissue such as monocytes and dendritic cells where they form heterodimers that can recognize triacylated lipoproteins.1) Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77. |
| Immunogen | TLR1 antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human TLR1. The immunogen is located within amino acids 160 - 210 of TLR1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TLR1 antibody can be used for detection of TLR1 by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TLR1 |
|---|---|
| Alternative Names | TLR1 Antibody: TIL CD281 rsc786 TIL. LPRS5 KIAA0012 Toll-like receptor 1 TIL |
| Molecular Weight(MW) | Predicted: 86 kDa Observed: 90 kDa |
Application
Western blot analysis of TLR1 in mouse spleen lysate with TLR1 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
Application
Immunofluorescence of TLR1 in Mouse Spleen tissue
Application
Immunohistochemistry of TLR1 in mouse spleen tissue with TLR1 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1406 - Mouse Spleen Tissue Lysate |
|---|---|
| Application Notes | TLR1 antibody can be used for detection of TLR1 by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TLR1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TLR1 Antibody is supplied in PBS containing 0.02% sodium azide. |
