Application
Western blot analysis of TLR2 in A20 cell lysates with TLR2 antibody at 1 μg/mL in the presence (A) and absence (B) of its blocking peptide.Application
Immunofluorescence of TLR2 in Human Spleen tissue with TLR2 antibody at 10 μg/mL.Application
Immunohistochemical staining of human spleen cells using TLR2 antibody at 2 μg/mL.| Product Name | TLR2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TLR2 Antibody: TIL4, CD282, TIL4, Toll-like receptor 2 |
| Product description | TLR2 Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-κB. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer can recognize a variety of bacterial and mycoplasma lipoproteins respectively.1) Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77. |
| Immunogen | TLR2 antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human TLR2. The immunogen is located within the first 50 amino acids of TLR2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TLR2 antibody can be used for detection of TLR2 by Western blot at 1 μg/mL. Despite its predicted molecular weight, TLR2 runs at ~100 kDa in SDS-PAGE. This polyclonal antibody can also detect TLR2 by immunohistochemistry at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TLR2 |
|---|---|
| Alternative Names | TLR2 Antibody: TIL4 CD282 TIL4 Toll-like receptor 2 |
| Sequence Similarities | 3) Cat. No. 10-901 - Human Spleen Tissue Slide |
Application
Western blot analysis of TLR2 in A20 cell lysates with TLR2 antibody at 1 μg/mL in the presence (A) and absence (B) of its blocking peptide.
Application
Immunofluorescence of TLR2 in Human Spleen tissue with TLR2 antibody at 10 μg/mL.
Application
Immunohistochemical staining of human spleen cells using TLR2 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | TLR2 antibody can be used for detection of TLR2 by Western blot at 1 μg/mL. Despite its predicted molecular weight, TLR2 runs at ~100 kDa in SDS-PAGE. This polyclonal antibody can also detect TLR2 by immunohistochemistry at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TLR2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TLR2 Antibody is supplied in PBS containing 0.02% sodium azide. |
