Application
Western blot analysis of TLR8 in Daudi cell lysates with TLR8 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.Application
Immunofluorescence of TLR8 in Daudi cells with TLR8 antibody at 10 μg/mL.Application
Immunocytochemistry of TLR8 in Daudi cells with TLR8 antibody at 2 μg/mL.| Product Name | TLR8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TLR8 Antibody: CD288, Toll-like receptor 8 |
| Product description | TLR8 Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. Like TLR7, TLR8 is localized to endosomal or lysosomal compartments and stimulates the innate immune response after activation by guanosine- and uridine-rich single-stranded RNA. Human but not murine TLR8 confers responsiveness to the antiviral compound R-848.1) Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77. |
| Immunogen | TLR8 antibody was raised against a peptide corresponding to 16 amino acids near the middle of human TLR8. The immunogen is located within amino acids 790 - 840 of TLR8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| TLR8 antibody can be used for detection of TLR8 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TLR8 |
|---|---|
| Alternative Names | TLR8 Antibody: CD288 Toll-like receptor 8 |
| Molecular Weight(MW) | Predicted: 112 kDa Observed: 107 kDa |
Application
Western blot analysis of TLR8 in Daudi cell lysates with TLR8 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Application
Immunofluorescence of TLR8 in Daudi cells with TLR8 antibody at 10 μg/mL.
Application
Immunocytochemistry of TLR8 in Daudi cells with TLR8 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | TLR8 antibody can be used for detection of TLR8 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TLR8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TLR8 Antibody is supplied in PBS containing 0.02% sodium azide. |
