Application
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.Application
Antibody used in IHC on Human Skeletal Muscle at 5.0 ug/ml.| Product Name | TNNI1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TNNI1, DKFZp451O223, TNN1, SSTNI |
| Product description | Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Each troponin-tropomyosin complex contains 1 molecule from each of the 3 troponins: T, C, and I. The troponin I (TNI) subfamily contains three genes: TNI-skeletal-fast-twitch, TNI-skeletal-slow-twitch, and TNI-cardiac. TNNI1 prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.1) Ralli, S., (2005) Am. Heart J. 150 (6), 1220-1227. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TNNI1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| TNNI1 antibody can be used for detection of TNNI1 by ELISA at 1:62500. TNNI1 antibody can be used for detection of TNNI1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNNI1 |
|---|---|
| Alternative Names | TNNI1 DKFZp451O223 TNN1 SSTNI |
| Molecular Weight(MW) | 21 kDa |
Application
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Skeletal Muscle at 5.0 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | TNNI1 antibody can be used for detection of TNNI1 by ELISA at 1:62500. TNNI1 antibody can be used for detection of TNNI1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store TNNI1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
