Application
(LEFT)Western blot analysis of TPPP3 Antibody in WiDr cell tissue lysates (35ug/lane).TPPP3 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of TPPP3 Antibody in mouse cerebellum tissue lysates (35ug/lane).TPPP3 (arrow) was detected using the purified Pab.Application
TPPP3 Antibody IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TPPP3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tubulin polymerization-promoting protein family member 3, TPPP/p20, TPPP3 |
| Product description | TPPP3 binds tubulin and has microtubule bundling activity. TPPP3 may play a role in cell proliferation and mitosis.1) Zhou, W., et al. Mol. Cell. Biochem. 333 (1-2), 91-98 (2010) Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009) Vincze, O., et al. Biochemistry 45(46):13818-13826(2006) |
| Immunogen | This TPPP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-146 amino acids from the C-terminal region of human TPPP3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TPPP3 |
|---|---|
| Alternative Names | Tubulin polymerization-promoting protein family member 3 TPPP/p20 TPPP3 |
| Molecular Weight(MW) | 19 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
(LEFT)Western blot analysis of TPPP3 Antibody in WiDr cell tissue lysates (35ug/lane).TPPP3 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of TPPP3 Antibody in mouse cerebellum tissue lysates (35ug/lane).TPPP3 (arrow) was detected using the purified Pab.
Application
TPPP3 Antibody IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
