Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).Application
TPTEa Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | TPTEa Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Putative tyrosine-protein phosphatase TPTE, Cancer/testis antigen 44, CT44, Transmembrane phosphatase with tensin homology, Tumor antigen BJ-HCC-5, TPTE |
| Product description | Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP-dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.1) Hattori, M., et al., Nature 405(6784):311-319 (2000). |
| Immunogen | This TPTEa antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-74 amino acids from the N-terminal region of human TPTEa. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TPTE |
|---|---|
| Alternative Names | Putative tyrosine-protein phosphatase TPTE Cancer/testis antigen 44 CT44 Transmembrane phosphatase with tensin homology Tumor antigen BJ-HCC-5 TPTE |
| Molecular Weight(MW) | 64 kDa |
Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Application
TPTEa Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
