Application
Western blot analysis of Translin in rat lung tissue lysate with Translin antibody at (A) 0.5 and (B) 1 μg/mLApplication
Immunofluorescence of Translin in human lung tissue with Translin antibody at 20 μg/mL.Application
Immunohistochemistry of Translin in human lung tissue with Translin antibody at 5 μg/mL.| Product Name | Translin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Translin Antibody: C3PO, RCHF1, TBRBP, TRSLN, BCLF-1, REHF-1, Translin, Component 3 of promoter of RISC, C3PO |
| Product description | Translin Antibody: Translin is a DNA-binding protein which specifically recognizes conserved target sequences at the breakpoint junction of chromosomal translocations. Translin polypeptides form a multimeric structure that is responsible for its DNA-binding activity. Recombination-associated motifs and translin-binding sites are present at recombination hotspots and may serve as indicators of breakpoints in genes which are fused by translocations. These binding activities may play a crucial role in chromosomal translocation in lymphoid neoplasms. Recent evidence suggests that in combination with a binding partner Trax, Translin binds RNA and mediates trafficking in neurons.1) Aoki K, Susuki K, Sugano T, et al. A novel gene, Translin, encodes a recombination hotspot binding protein associated with chromosomal translocations. Nat. Genet. 1995; 10:167-74. |
| Immunogen | Translin antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human Translin. The immunogen is located within the last 50 amino acids of Translin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Translin antibody can be used for detection of Translin by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TSN |
|---|---|
| Alternative Names | Translin Antibody: C3PO RCHF1 TBRBP TRSLN BCLF-1 REHF-1 Translin Component 3 of promoter of RISC C3PO |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | At least two isoforms of Translin are known to exist; this antibody will detect both. |
Application
Western blot analysis of Translin in rat lung tissue lysate with Translin antibody at (A) 0.5 and (B) 1 μg/mL
Application
Immunofluorescence of Translin in human lung tissue with Translin antibody at 20 μg/mL.
Application
Immunohistochemistry of Translin in human lung tissue with Translin antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1462 - Rat Lung Tissue Lysate |
|---|---|
| Application Notes | Translin antibody can be used for detection of Translin by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Translin antibody can be stored at 4 ˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Translin Antibody is supplied in PBS containing 0.02% sodium azide. |
