Application
Western blot analysis of TRAP in mouse brain tissue lysate with TRAP antibody at 1 μg/mL.Application
Immunofluorescence of TRAP in human liver tissue with TRAP antibody at 20 μg/mL.| Product Name | TRAP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRAP Antibody: TRAP, SPENCDI, Tartrate-resistant acid phosphatase type 5, Tartrate-resistant acid ATPase, TR-AP |
| Product description | TRAP Antibody: TRAP, also known as uteroferrin, is an iron containing, glycosylated, acid phosphatase. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. Along with the related protein ACP2, TRAP mediates the removal of mannose 6-phosphate residues from proteins targeted to lysosomes. TRAP is present in brain at low levels, but is expressed at a much higher level in liver.1) Baumbach GA, Saunders PT, Bazer FW, et al. Uteroferrin has N-apsaragine-linked high mannose-type oligosaccharaides that contain mannose 6-phosphate. Proc. Natl. Acad. Sci. USA 1984; 81:2985-9. |
| Immunogen | TRAP antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human TRAP. The immunogen is located within the last 50 amino acids of TRAP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| TRAP antibody can be used for detection of TRAP by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACP5 |
|---|---|
| Alternative Names | TRAP Antibody: TRAP SPENCDI Tartrate-resistant acid phosphatase type 5 Tartrate-resistant acid ATPase TR-AP |
| Sequence Similarities | 2) Cat. No. 10-201 - Human Liver Tissue Slide |
| Entrez Gene | 54 |
|---|---|
| Protein Accession | NP_001104505 |
Application
Western blot analysis of TRAP in mouse brain tissue lysate with TRAP antibody at 1 μg/mL.
Application
Immunofluorescence of TRAP in human liver tissue with TRAP antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | TRAP antibody can be used for detection of TRAP by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TRAP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TRAP Antibody is supplied in PBS containing 0.02% sodium azide. |
