Application
Western blot analysis of TREX1 in human spleen tissue lysate with TREX1 antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of TREX1 in Human Spleen tissue with TREX1 antibody at 20 μgg/mL.Application
Immunohistochemistry of TREX1 in human spleen tissue with TREX1 antibody at 2.5 μg/mL.| Product Name | TREX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TREX1 Antibody: CRV, AGS1, DRN3, HERNS, Three prime repair exonuclease 1, DNase III |
| Product description | TREX1 Antibody: Trex1 is the major human 3' to 5' exonuclease which is required for checkpoint signaling after DNA damage. It is ubiquitously expressed, binds to single stranded DNA coated with replication protein A that accumulates at sites of DNA damage and recruits the ataxia telangiectasia and Rad3 related protein (ATR), a checkpoint kinase, to sites of DNA damage and replication stress. Trex1 is required for ATR expression. This gene uses two different open reading frames. The upstream ORF encodes proteins which interact with ATR and localize to intranuclear foci induced by DNA damage and are essential components of the DNA damage checkpoint. The downstream ORF encodes proteins with 3' to 5' exonuclease activity and may be a subunit of human DNA polymerase III. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, and Cree encephalitis.1) Mazur DJ and Perrino FW. Identification and expression of the TREX1 and TREX2 cDNA sequences encoding mammalian 3→5 exonucleases. J. Biol. Chem. 1999; 274:19655-60. |
| Immunogen | TREX1 antibody was raised against a 15 amino acid synthetic peptide near the center of human TREX1. The immunogen is located within amino acids 220 - 270 of TREX1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TREX1 antibody can be used for detection of TREX1 by Western blot at 0.5 and 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TREX1 |
|---|---|
| Alternative Names | TREX1 Antibody: CRV AGS1 DRN3 HERNS Three prime repair exonuclease 1 DNase III |
| Molecular Weight(MW) | Predicted: 33, 35, 41 kDa Observed: 31 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | TREX1 antibody will not cross-react with the related protein TREX2. At least three isoforms of TREX1 are known to exist; this antibody will recognize all three isoforms. |
Application
Western blot analysis of TREX1 in human spleen tissue lysate with TREX1 antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of TREX1 in Human Spleen tissue with TREX1 antibody at 20 μgg/mL.
Application
Immunohistochemistry of TREX1 in human spleen tissue with TREX1 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | TREX1 antibody can be used for detection of TREX1 by Western blot at 0.5 and 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TREX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TREX1 Antibody is supplied in PBS containing 0.02% sodium azide. |
