Application
Antibody used in WB on Transfected 293T at 1.25 ug/ml.| Product Name | TRIM21 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRIM21, RNF81, RO52, SSA, SSA1, Ro/SSA |
| Product description | TRIM21 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TRIM21 is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus.This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Yamochi, T., (2008) Biochem. Biophys. Res. Commun. 370 (1), 195-199. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TRIM21. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TRIM21 antibody can be used for detection of TRIM21 by ELISA at 1:312500. TRIM21 antibody can be used for detection of TRIM21 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TRIM21 |
|---|---|
| Alternative Names | TRIM21 RNF81 RO52 SSA SSA1 Ro/SSA |
| Molecular Weight(MW) | 54 kDa |
Application
Antibody used in WB on Transfected 293T at 1.25 ug/ml.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | TRIM21 antibody can be used for detection of TRIM21 by ELISA at 1:312500. TRIM21 antibody can be used for detection of TRIM21 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store TRIM21 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
