Application
Immunocytochemistry of TRIM25 in HeLa cells with TRIM25 antibody at 20 μg/mL.Application
Immunofluorescence of TRIM25 in HeLa cells with TRIM25 antibody at 20 μg/mL.Application
Western blot analysis of TRIM25 in HeLa cell lysate in (A) the absence and (B) presence of blocking peptide with TRIM25 antibody at 1 μg/mL.| Product Name | TRIM25 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRIM25 Antibody: EFP, Z147, RNF147, ZNF147, EFP, Estrogen-responsive finger protein |
| Product description | TRIM25 Antibody: TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.1) Inoue S, Orimo A, Hosoi T, et al. Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein. Proc. Natl. Acad. Sci. USA1993; 11117-20. |
| Immunogen | TRIM25 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human TRIM25. The immunogen is located within amino acids 520 - 570 of TRIM25. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| TRIM25 antibody can be used for detection of TRIM25 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRIM25 |
|---|---|
| Alternative Names | TRIM25 Antibody: EFP Z147 RNF147 ZNF147 EFP Estrogen-responsive finger protein |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunocytochemistry of TRIM25 in HeLa cells with TRIM25 antibody at 20 μg/mL.
Application
Immunofluorescence of TRIM25 in HeLa cells with TRIM25 antibody at 20 μg/mL.
Application
Western blot analysis of TRIM25 in HeLa cell lysate in (A) the absence and (B) presence of blocking peptide with TRIM25 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | TRIM25 antibody can be used for detection of TRIM25 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TRIM25 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TRIM25 Antibody is supplied in PBS containing 0.02% sodium azide. |
