Application
Western blot analysis in A549,293,HepG2 cell line lysates (35ug/lane).Application
TRIM4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | TRIM4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E3 ubiquitin-protein ligase TRIM4, 632-, RING finger protein 87, Tripartite motif-containing protein 4, TRIM4, RNF87 |
| Product description | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternatively spliced transcript variants that encode different isoforms have been described.1) Kasyapa, C., et al. Proteomics 9(16):3979-3988(2009) |
| Immunogen | This TRIM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-141 amino acids from the N-terminal region of human TRIM4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRIM4 |
|---|---|
| Alternative Names | E3 ubiquitin-protein ligase TRIM4 632- RING finger protein 87 Tripartite motif-containing protein 4 TRIM4 RNF87 |
| Molecular Weight(MW) | 57 kDa |
Application
Western blot analysis in A549,293,HepG2 cell line lysates (35ug/lane).
Application
TRIM4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
