Application
Western blot analysis of TRIM71 in human brain tissue lysate with TRIM71 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of TRIM71 in Human Brain cells with TRIM71 antibody at 20 μg/mL.Application
Immunohistochemistry of TRIM71 in human brain tissue with TRIM71 antibody at 5 μg/mL.| Product Name | TRIM71 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRIM71 Antibody: LIN41, LIN-41, LIN41, E3 ubiquitin-protein ligase TRIM71, Protein lin-41 homolog |
| Product description | TRIM71 Antibody: TRIM71 is the mammalian ortholog to the C. elegans heterochronic protein Lin41, a protein that is thought to be important in postembryonic development, and is genetically and biochemically downstream of both Shh and Fgf signaling pathways. Similar to its C. elegans homolog, TRIM71 is a target of the microRNA let-7 and is likely to play a role in mammalian development. Recent experiments have indicated that TRIM71 is an E3 ubiquitin ligase and can interact with the Dicer and the Argonaute proteins Ago1, Ago2, and Ago4. Overexpression and depletion of TRIM71 led to inverse changes in Ago2 protein levels, suggesting TRIM71 can regulate Ago2 turnover. Finally, TRIM71 cooperates with the pluripotency factor Lin-28 in regulating let-7.1) Lancman JJ, Caruccio NC, Harfe BD, et al. Analysis of the regulation of lin-41 during chick and mouse limb development. Dev. Dyn.2005; 234:948-60. |
| Immunogen | TRIM71 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human TRIM71. The immunogen is located within amino acids 680 - 730 of TRIM71. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TRIM71 antibody can be used for detection of TRIM71 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRIM71 |
|---|---|
| Alternative Names | TRIM71 Antibody: LIN41 LIN-41 LIN41 E3 ubiquitin-protein ligase TRIM71 Protein lin-41 homolog |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Entrez Gene | 131405 |
|---|---|
| Protein Accession | NP_001034200 |
Application
Western blot analysis of TRIM71 in human brain tissue lysate with TRIM71 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of TRIM71 in Human Brain cells with TRIM71 antibody at 20 μg/mL.
Application
Immunohistochemistry of TRIM71 in human brain tissue with TRIM71 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | TRIM71 antibody can be used for detection of TRIM71 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TRIM71 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TRIM71 Antibody is supplied in PBS containing 0.02% sodium azide. |
