Application
Western blot analysis of TSC1 in EL4 cell lysate with TSC1 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.Application
Immunofluorescence of TSC1 in L1210 cells with TSC1 antibody at 10 μg/mL.Application
Immunocytochemistry of TSC1 in EL4 cells with TSC1 antibody at 2 μg/mL| Product Name | TSC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TSC1 Antibody: LAM, TSC, KIAA0243, Hamartin, Tuberous sclerosis 1 protein |
| Product description | TSC1 Antibody: Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.1) Shamji AF, Ngheim P, and Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Mol. Cell 2003; 12:271-80. |
| Immunogen | TSC1 antibody was raised against a 15 amino acid synthetic peptide from the middle region of human TSC1. The immunogen is located within amino acids 220 - 270 of TSC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| TSC1 antibody can be used for the detection of TSC1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TSC1 |
|---|---|
| Alternative Names | TSC1 Antibody: LAM TSC KIAA0243 Hamartin Tuberous sclerosis 1 protein |
| Molecular Weight(MW) | Predicted: 128 kDa Observed: 135 kDa |
| Sequence Similarities | 3) Cat. No. 17-204 - L1210 Cell Slide |
| Tissue Specificity | At least two isoforms of TSC1 are known to exist; this antibody will detect both isoforms. TSC1 antibody is predicted to not cross-react with TSC2. |
Application
Western blot analysis of TSC1 in EL4 cell lysate with TSC1 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
Application
Immunofluorescence of TSC1 in L1210 cells with TSC1 antibody at 10 μg/mL.
Application
Immunocytochemistry of TSC1 in EL4 cells with TSC1 antibody at 2 μg/mL| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | TSC1 antibody can be used for the detection of TSC1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TSC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TSC1 Antibody is supplied in PBS containing 0.02% sodium azide. |
