Application
Western blot analysis of TSC2 in L1210 cell lysate with TSC2 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.Application
Western blot analysis of TSC2 in Jurkat cell lysate with TSC2 antibody at (A) 1 and (B) 2 μg/mL..| Product Name | TSC2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TSC2 Antibody: LAM, TSC4, Tuberin, Tuberous sclerosis 2 protein |
| Product description | TSC2 Antibody: Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 complex decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing that overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. At least three isoforms of TSC2 exist.1) Shamji AF, Ngheim P, and Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Mol. Cell 2003; 12:271-80. |
| Immunogen | TSC2 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human TSC2. The immunogen is located within amino acids 1450 - 1500 of TSC2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TSC2 antibody can be used for the detection of TSC2 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TSC2 |
|---|---|
| Alternative Names | TSC2 Antibody: LAM TSC4 Tuberin Tuberous sclerosis 2 protein |
| Molecular Weight(MW) | Predicted: 200 kDa Observed: 200 kDa |
| Sequence Similarities | 2) Cat. No. 1205 - Jurkat Cell Lysate |
Application
Western blot analysis of TSC2 in L1210 cell lysate with TSC2 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
Application
Western blot analysis of TSC2 in Jurkat cell lysate with TSC2 antibody at (A) 1 and (B) 2 μg/mL..| Positive Control | 1) Cat. No. 1284 - L1210 Cell Lysate |
|---|---|
| Application Notes | TSC2 antibody can be used for the detection of TSC2 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TSC2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TSC2 Antibody is supplied in PBS containing 0.02% sodium azide. |
